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. 2017 Nov 27;293(2):403–411. doi: 10.1074/jbc.M117.805739

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Figure 3. — Purified IL-36 cytokines retain their biological activity as measured in secreted CXCL1 assay. Human HaCaT keratinocyte cell line was treated for 24 h with IL-36α (left panel) or IL-36γ (middle panel) at the indicated concentrations. Measurement of secreted CXCL1 shows similar potencies between the two cytokines. HaCaT keratinocyte cells were pretreated with IL-36Ra (right panel) at the indicated concentrations for 15 min and then overlaid with 60 ng/ml of IL-36γ for 24 h. Measurement of secreted CXCL1 shows IL-36Ra potency is consistent with reported values (15, 16). Averages of triplicate measurements are shown in the plot. Standard deviations are represented as error bars. The data are fit using Equation 1 to obtain EC₅₀.