Figure 2.

R123, BER, and PQ are substrates of the Cdr6/Roa1 transporter. A, left, comparison of growth by spot dilution assays for C. albicans WT, CDR6/ROA1 null mutant NKKY101, CDR6/ROA1 revertant NKKY102, and CDR6/ROA1 overexpression strain NKKY103 cells onto R123, BER, and PQ at the indicated concentrations in YEPD agar plates. Right, MIC₅₀ values for these strains, obtained by use of a broth microdilution assay, starting with the indicated concentration of xenobiotics in the first well in YEPD medium, as described under “Experimental procedures.” For each strain, optical densities were averaged for duplicate measurements, and relative growths (as compared with drug-free growth) were displayed with color as indicated with the color bar using three-color heat maps. B, R123 accumulation assay in WT, NKKY101, NKKY102, and NKKY103 strains cells using FACS. n = 10,000 cells for each sample. C, BER accumulation assay in WT, NKKY101, NKKY102, and NKKY103 strains cells using FACS. n = 10,000 cells for each sample.