Figure 3.

CDR6/ROA1 deletion leads to resistance toward azoles and low accumulation of azoles. A, comparison of growth by spot dilution assays between WT, NKKY101, NKKY102, and NKKY103 strains. A 5-fold serial dilution of each strain was spotted on YEPD agar plates containing the triazoles (FLC and ITR) and imidazoles (KTC and MCZ) at the indicated concentrations and grown for 48 h at 30 °C. B, drug resistance profiles of WT, NKKY101, NKKY102, and NKKY103 strains on FLC by a filter disk assay as described under “Experimental procedures.” C, growth curve of WT, NKKY101, NKKY102, and NKKY103 strains in YEPD medium in the absence (left) and presence (right) of FLC. D, [³H]FLC accumulation levels in C. albicans WT, NKKY101, NKKY102, and NKKY103 strains were measured at 24 h in the absence of glucose after cells were carbon-starved for 3 h. E, [³H]FLC accumulation levels in C. albicans WT, NKKY101, NKKY102, and NKKY103 strains were measured at 24 h in the presence of 2% glucose after cells were carbon-starved for 3 h. All experiments were performed in biological triplicate, and the results are shown as means ± S.D. (error bars). A statistical significance value (**, p ≤ 0.01; *, p ≤ 0.05; ns, not significantly different) was employed using unpaired Student's t test.