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. 2017 Nov 21;293(2):466–483. doi: 10.1074/jbc.M117.812834

Figure 5.

Figure 5.

EPA and DHA administration to a Western diet lowers select murine cardiac mitochondrial enzyme activities. A, complex I activity, measured by NADH oxidation for mice consuming a control (CON), Western diet (WD), WD + EPA, and WD + DHA. B, complex II activity, assessed by dichlorophenolindophenol reduction. C, complex III activity, assayed by cytochrome c reduction. D, complex IV activity, measured by cytochrome c oxidation. E, complex V activity, measured by the oxidation of NADH. F, complex I + III activity measured by NADH oxidation coupled to cytochrome c reduction. G, complex II + III activity assayed by succinate oxidation coupled to cytochrome c reduction. Activities were determined relative to total protein content and then normalized to citrate synthase (CS) activity. Data are the average ± S.D. from 5 to 6 independent experiments. Asterisks indicate significance from control (*, p < 0.05; **, p < 0.01). Horizontal bars with asterisks indicate significance between treatments.