Human FHOD1 accelerates assembly of Acanthamoeba actin, but not rabbit skeletal actin.
A, assembly of 2 μm
A. castellanii actin (10% pyrene-labeled) alone or in the presence of 40 nm human FHOD1. B, assembly of 2 μm actin from Acanthamoeba or rabbit skeletal muscle (10% pyrene-labeled) alone or in the presence of 40 nm human FHOD1. C, assembly of 2 μm actin from Acanthamoeba or rabbit skeletal muscle (10% pyrene-labeled) alone or in the presence of 8 nm
Drosophila Fhod. D, quantification of actin assembly rates from C. The data are means ± standard deviation from three independent experiments.