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. 2017 Dec 6;293(2):541–556. doi: 10.1074/jbc.RA117.000504

Figure 8.

Figure 8.

Conversion of 17α-OOH pregnenolone to DHEA by human P450 17A1. A, HPLC of standard 17α-OOH pregnenolone, 17α-OH pregnenolone, and DHEA, showing tR for elution. B, chromatograms of reaction of 25 μm 17α-OOH pregnenolone without (green) or with (blue) 60 nm human P450 17A1 after 150 s. Absorbance (relative units) was integrated over the UV range (200–350 nm). See “Experimental procedures” for other details regarding chromatography. C, rate of conversion of 17α-OOH pregnenolone to DHEA, with or without human b5. D, rate of conversion of 17α-OOH progesterone to Andro. The points indicate single assays done at each time.