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Increased HA content leads to mechanically soft hydrogels. A, stiffness measurements via rheometer of cross-linked hydrogels with 0–25% total HA content. B, stiffness measurement via rheometer of hyal-treated cross-linked hydrogels with 0–25% total HA content. C, stiffness measurement via AFM of cross-linked hydrogels with 0–25% total HA content. Stiffness is expressed as Young's modulus in Pa. The data show means ± S.E. *, p < 0.05 versus control by unpaired t test.
