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. 2017 Nov 20;293(2):717–730. doi: 10.1074/jbc.M117.815381

Figure 5.

Figure 5.

8-CPT-AM attenuates VEGF-Erk1/2 signal transduction. A, immunoblotting was used to detect phospho-Tyr-1175 and total VEGFR2, phospho and total Erk1/2, and phospho-Ser-473 and total AKT changes in BREC pre-treated with 8-CPT-AM (1 μm) for 30 min followed by 15 min of VEGF (50 ng/ml). B, VEGFR2 Tyr-1175 phosphorylation was reduced by 8-CPT-AM when added prior to VEGF, n ≥ 12. C, 8-CPT-AM decreased Erk1 and D, Erk2 phosphorylation, n ≥ 10. E, both VEGF and 8-CPT-AM increase AKT phosphorylation, n ≥ 4. Values are mean ± S.D. ####, p < 0.0001 or ###, p < 0.001 or ##, p < 0.005 or #, p < 0.05 compared with control and ****, p < 0.0001 or **, p < 0.01 compared with VEGF.