Figure S1.

IDH1R132H decreased proliferation and migration in LNCAP cells. (A) The representative transfection efficiency of virus observation in RWPE-1 and LNCAP cells under fluorescence microscope. (B) The wild-type and mutant IDH1 protein and mRNA expression levels of LNCAP cells stably expressing IDH1R132H, wild-type, and empty vector were tested by Western blot and qRT-PCR. (C, D) MTS and Transwell migration assays were used to identify the abilities of proliferation and migration in IDH1R132H, IDH1WT, and VECTOR-expressing LNCAP cells. (E) Growth curves were used to test tumors formation of subcutaneous xenograft assay. (F) The weight of tumors was measured at harvest time (n = 6 per group). Data in C and D represent mean ± SD of at least three independent replicates. P values: *P < .05