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Designed primers for arginine phenyl alanine related peptide-3 (RFRP3) and β-actin genes and their amplification reaction conditions
| Gene | Primer sequencing (5ˊ-3ˊ) | Amplicon length (bp) |
|---|---|---|
| RFRP3 | F: AAGACACTGGCTGGTTTG | 192 |
| R: TTGAAGGACTGGCTGGAG | ||
| β-actin | F: CCACACTTTCTACAATGAGC | 169 |
| R: ATACAGGGACAACACAGC | ||
