Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used for real-time quantitative polymerase chain reaction
| Primer | Primer sequencing (5ˊ-3ˊ) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|
| Kiss-1 | F:TGCTGCTTCTCCTCTGTG | 106 | 59 |
| R:ACGAGTTCCTGGGGTCC | |||
| β-actin | F:CCATCTATGAGGGTTACGC | 105 | 60 |
| R:TGTAGCCACGCTCGGTC | |||
