Table I.

Primers used for quantitative polymerase chain reaction.

Gene	Length, bp	Direction	Primer sequence
Wnt2b	102	Forward	5′-GCCGTGTCATGCTCAGAA-3′
		Reverse	5′-GTGGACTACCCCTGCTGATG-3′
Wnt3	104	Forward	5′-CTCGCTGGCTACCCAATTT-3′
		Reverse	5′-GCCCAGAGATGTGTACTGCTG-3′
Wnt3a	218	Forward	5′-CATGAACCGCCACAACAAC-3′
		Reverse	5′-TGGCACTTGCACTTGAGGT-3′
Wnt5a	200	Forward	5′-ATTGTACTGCAGGTGTACCTTAAAAC-3′
		Reverse	5′-CCCCCTTATAAATGCAACTGTTC-3′
Wnt5b	175	Forward	5′-CTGCTGCTGCTGTTCACG-3′
		Reverse	5′-CACCGGGTTCAAAGCTAATG-3′
Wnt7b	159	Forward	5′-CGCCTCATGAACCTGCATA-3′
		Reverse	5′-GCTGCATCCGGTCCTCTA-3′
Wnt11	137	Forward	5′-TGTGCTATGGCATCAAGTGG-3′
		Reverse	5′-CAGTGTTGCGTCTGGTTCAG-3′
β-actin	147	Forward	5′-CCAACCGCGAGAAGATGA-3′
		Reverse	5′-CCAGAGGCGTACAGGGATAG-3′
FZD2	157	Forward	5′-GGTGTCGGTGGCCTACAT-3′
		Reverse	5′-GAGAAGCGCTCGTTGCAC-3′
FZD6	201	Forward	5′-TGGGTTGGAAGCAAAAAGAC-3′
		Reverse	5′-TCTTCGACTTTCACTGATTGGA-3′
FZD7	210	Forward	5′-GCCAGCTTGTGCCTAATAGAA-3′
		Reverse	5′-AGCCGGGAGAAACTCACAG-3′
β-catenin	204	Forward	5′-CTTACACCCACCATCCCACT-3′
		Reverse	5′-CCTCCACAAATTGCTGCTGT-3′
APC	209	Forward	5′-GCCCCTGACCAAAAAGGAAC-3′
		Reverse	5′-TGGCAGCAACAGTCCCACTA-3′
GSK3β	221	Forward	5′-CAAGCCAAACTTTGTGACTCAG-3′
		Reverse	5′-TATCAGGATCCAGCAAGAGGTT-3′
Axin1	130	Forward	5′-AGCCGTGTCGGACATGGA-3′
		Reverse	5′-AAGTAGTACGCCACAACGATGCT-3′
Axin2	210	Forward	5′-TGTGAGGTCCACGGAAACTG-3′
		Reverse	5′-CGTCAGCGCATCACTGGATA-3′
Cyclin D1	166	Forward	5′-TCAAATGTGTGCAGAAGGAGGT-3′
		Reverse	5′-GACAGGAAGCGGTCCAGGTA-3′

APC, adenomatous polyposis coli; FZD, frizzled; GSK3β, glycogen synthase kinase 3β.