Table I.
Gene | Length, bp | Direction | Primer sequence |
---|---|---|---|
Wnt2b | 102 | Forward | 5′-GCCGTGTCATGCTCAGAA-3′ |
Reverse | 5′-GTGGACTACCCCTGCTGATG-3′ | ||
Wnt3 | 104 | Forward | 5′-CTCGCTGGCTACCCAATTT-3′ |
Reverse | 5′-GCCCAGAGATGTGTACTGCTG-3′ | ||
Wnt3a | 218 | Forward | 5′-CATGAACCGCCACAACAAC-3′ |
Reverse | 5′-TGGCACTTGCACTTGAGGT-3′ | ||
Wnt5a | 200 | Forward | 5′-ATTGTACTGCAGGTGTACCTTAAAAC-3′ |
Reverse | 5′-CCCCCTTATAAATGCAACTGTTC-3′ | ||
Wnt5b | 175 | Forward | 5′-CTGCTGCTGCTGTTCACG-3′ |
Reverse | 5′-CACCGGGTTCAAAGCTAATG-3′ | ||
Wnt7b | 159 | Forward | 5′-CGCCTCATGAACCTGCATA-3′ |
Reverse | 5′-GCTGCATCCGGTCCTCTA-3′ | ||
Wnt11 | 137 | Forward | 5′-TGTGCTATGGCATCAAGTGG-3′ |
Reverse | 5′-CAGTGTTGCGTCTGGTTCAG-3′ | ||
β-actin | 147 | Forward | 5′-CCAACCGCGAGAAGATGA-3′ |
Reverse | 5′-CCAGAGGCGTACAGGGATAG-3′ | ||
FZD2 | 157 | Forward | 5′-GGTGTCGGTGGCCTACAT-3′ |
Reverse | 5′-GAGAAGCGCTCGTTGCAC-3′ | ||
FZD6 | 201 | Forward | 5′-TGGGTTGGAAGCAAAAAGAC-3′ |
Reverse | 5′-TCTTCGACTTTCACTGATTGGA-3′ | ||
FZD7 | 210 | Forward | 5′-GCCAGCTTGTGCCTAATAGAA-3′ |
Reverse | 5′-AGCCGGGAGAAACTCACAG-3′ | ||
β-catenin | 204 | Forward | 5′-CTTACACCCACCATCCCACT-3′ |
Reverse | 5′-CCTCCACAAATTGCTGCTGT-3′ | ||
APC | 209 | Forward | 5′-GCCCCTGACCAAAAAGGAAC-3′ |
Reverse | 5′-TGGCAGCAACAGTCCCACTA-3′ | ||
GSK3β | 221 | Forward | 5′-CAAGCCAAACTTTGTGACTCAG-3′ |
Reverse | 5′-TATCAGGATCCAGCAAGAGGTT-3′ | ||
Axin1 | 130 | Forward | 5′-AGCCGTGTCGGACATGGA-3′ |
Reverse | 5′-AAGTAGTACGCCACAACGATGCT-3′ | ||
Axin2 | 210 | Forward | 5′-TGTGAGGTCCACGGAAACTG-3′ |
Reverse | 5′-CGTCAGCGCATCACTGGATA-3′ | ||
Cyclin D1 | 166 | Forward | 5′-TCAAATGTGTGCAGAAGGAGGT-3′ |
Reverse | 5′-GACAGGAAGCGGTCCAGGTA-3′ |
APC, adenomatous polyposis coli; FZD, frizzled; GSK3β, glycogen synthase kinase 3β.