Figure 3.

miR-204 directly inhibits TCF12 expression by targeting its 3′UTR. (A) Predicted binding sites of miR-204 in the 3′UTR of TCF12. Mutant-type was generated in the seed region of the 3′UTR of TCF12 by mutating 3 nucleotides that is recognized by miR-204. (B) Luciferase activity assay was performed in HeLa and C33A cells co-transfected with miR-204 mimics and a luciferase reporter containing the WT Mut 3′UTR of TCF12. (C) The expression of TCF12 in HeLa and C33A cells after 48 h transfection with miR-204 mimics and NC-mim was measured by western blot analysis. **P<0.01. TCF12, transcription factor 12; miR-204, microRNA-204′ 3′UTR, 3′untranslated region; mim-NC, mimic negative control; Mut, mutant; WT, wild-type.