Figure 3.

TT-1 induces changes in expression of mitochondrial-dependent proteins. (A and B) Quantitative reverse transcription polymerase chain reaction of apoptosis-associated genes in TT cells demonstrated significant (A) upregulation of Bax and (B) downregulation of Bcl-2 at the mRNA level. The data represent the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01 vs. the untreated group. (C) Western blot analysis confirmed the upregulation of Bax and downregulation of Bcl-2 at the protein level. Densitometric analysis of Bax (D) or Bcl-2 (E) protein levels. **P<0.01 vs. the control group. Western blots are representative blots from three independent experiments with similar results.