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. 2018 Jan 9;8:2644. doi: 10.3389/fmicb.2017.02644

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Copyright © 2018 Shukla, Rajpoot, Arora, Poddar, Swaminathan and Khanna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Additional physical characterization of E1E2_bv. (A) Sandwich ELISA demonstrating the presence of E1 and E2 proteins in the purified material. The purified material was captured using DENV-1 mAb (mAb E103: light brown bars; mAb E29: dark brown bars) and revealed using DENV-2 mAb 3H5-Eu^∗ conjugate. Inset depicts a schematic representation of the sandwich ELISA of mE1E2_bv VLPs. (B) DLS analysis of purified mE1E2_bv VLPs. The average diameter of the VLPs is shown at the top of the peak; the value in parenthesis indicates the proportion (in %) of these VLPs in the purified preparation. (C) EM picture of the VLPs after negative staining with uranyl acetate (the scale is shown on the bottom right).