Table 1.

Analysis of E-specific epitope integrity of the mE1E2_bv VLPs.

mAba	Serotype specificity	Epitope specificityb	ELISA OD450 nm c
			mE1E2bv VLPs	E1	E2	E3	E4
E103	DENV-1	EDIII LR	3.48	3.46	0.06	0.03	0.09
E24		EDIII	0.77	1.23	0.03	0.02	0.03
E37		EDIII	0.93	1.25	0.02	0.02	0.02
E29		EDIII	1.67	1.87	0.03	0.09	0.14
E12		EDIII	1.54	1.82	0.07	0.12	0.07
3H5	DENV-2	EDIII LR	3.45	0.14	3.46	0.06	0.11
106		EDIII LR	2.51	0.05	2.5	0.03	0.03
104		EDIII C-C LOOP	3.44	0.08	3.45	0.1	0.05
70		EDIII AS/LR	0.91	0.02	0.92	0.03	0.03
8A1	DENV-3	EDIII LR	0.06	0.04	0.07	1.39	0.05
E88	DENV-4	EDIII LR	0.08	0.06	0.04	0.07	2.93
E77	All four DENV serotypes	EDIII AS/LR	1.25	3.50	1.21	1.64	1.21
12C1.5		EDIII	3.76	3.66	3.86	3.21	3.01
4G2		FL	0.13	0.23	0.20	0.21	0.07
h-2J20		EDIII	3.03	3.67	3.46	1.67	1.08
h-3H4		EDII/III	1.13	0.11	0.16	0.15	0.12
h-1M7		FL	2.45	2.97	3.03	1.28	1.93
h-1N5		FL	0.37	0.95	0.45	0.19	0.13
h-DVC23.13		EDI/II	0.41	0.22	0.09	0.13	0.09
h-2K2		prM	0.01	0.02	0.02	0.01	0.02

^amAbs with ‘h’ prefix are of human origin; the rest are murine mAbs; these mAbs are described in the following references: Shrestha et al. (2010) (mAbs E12, E24, E29, E37 and E103); Henchal et al. (1982) (mAbs 3H5 and 4G2); Sukupolvi-Petty et al. (2010) (mAbs 106, 104, 70); Sukupolvi-Petty et al. (2013) (mAb E88); Wahala et al. (2010) (mAbs 8A1 and 12C1.5); Brien et al. (2010) (mAb E77); Smith et al. (2012) (mAbs 2J20 and 3H4); Smith et al. (2013) (mAbs 1M7 and 1N5); De Alwis et al. (2011) (mAb DVC23.13); De Alwis et al. (2014) (mAb 2K2).

^bAbbreviations are as follows: LR, lateral ridge; AS, A strand; FL, fusion loop.

^cData shown are the average of duplicate experiments.