Figure 7.

PD169316 reduces cell proliferation inhibition and apoptosis induced by lapatinib. NB4 cells were treated with 15 µM lapatinib for 24 h in the presence or absence of PD169316 or SP600125. (A) Cell viability was measured by CCK-8 assay. (B) Cell apoptosis was analyzed by flow cytometry. (C) The levels of Bax, PARP, cleaved caspase-3/9, and caspase-3/9 were measured by western blotting. Quantification analysis of western blotting, β-actin was served as a control. Data was expressed as means ± SD. *P<0.05 (A: 1 DMSO; 2 15 µM lapatinib; 3 20 µM lapatinib. D: 1 DMSO; 2 PD169316; 3 15 µM lapatinib; 4 PD169316+15 µM lapatinib).