Figure 2.

TASK-1 induces formation of CIC phenotypes in A549 cells. (A) Sphere growth of A549 cells transfected with TASK-1-expressing plasmids and empty vectors (mock) (scale bar, 100 µm). (B) Western blot analysis of CD133, OCT-4 and Nanog in A549 cells transfected with TASK-1-expressing plasmids and empty vectors (mock). β-actin was used as a loading control. Representative images of triplicate experiments are shown. TASK-1, TWIK-related acid-sensitive K(+); OCT-4, octamer-binding transcription factor 4.