Figure 4.

Overexpression of TASK-1 promotes migration of A549 cells. (A) Wound-healing assay of A549 cells transfected with TASK-1-expressing plasmids and empty vectors (mock). Images of the cell monolayers were captured directly after scraping and subsequent to 48 h of incubation. The wound healing rate was quantified by detecting the percentage of wound closure vs. that of the original wound. Values are expressed as the mean ± standard error of the mean (n=3). Scale bar=100 µm. (B) Western blot analysis of MMP-2 and MMP-9 expression in A549 cells transfected with TASK-1-expressing plasmids and empty vectors (mock). β-actin was used as a loading control. Representative images of triplicate experiments are shown. MMP, matrix metalloproteinase; TASK-1, TWIK-related acid-sensitive K(+).