Figure 2.

miR-144 inhibits the proliferation of osteosarcoma cells. MTT assay demonstrates the effect of miR-144 on proliferation of (A) MG63 and (B) U-2 OS cells. (C) Western blot analysis of proteins associated with cell proliferation in MG-63 cells. p53 and p21 act as cell cycle arrest genes, while PCNA is a biomarker of cell proliferation. (D) BrdU-incorporated flow cytometry showing proliferation data. ***P<0.01, n=3. PCNA, proliferating cell nuclear antigen; miR, microRNA; BrdU, 5-bromo-2′-deoxyuridine; FITC, fluorescein isothiocyanate.