Figure 5.

The role of mitogen-activated protein kinases in heat-stress-triggered apoptosis in neurons. F98 cells were pretreated with or without the indicated inhibitors prior exposure to heat stress or control heat treatment, and cells were further incubated for 12 h at 37°C. H₂O₂ was used as a positive control for ROS. (A and B) ROS quantity was assessed by 2′,7′-dichlorodihydrofluorescein diacetate staining; (A) images were obtained via laser scanning confocal microscopy and (B) analysis of the fluorescence intensity of ROS probes was performed using flow cytometry. (C) Analysis of apoptosis was performed through flow cytometry using Annexin V-fluorescein isothiocyanate/propidium iodide staining. ^#P<0.05 vs. HS group, *P<0.05 vs. control group. ROS, reactive oxygen species; HS, heat stress; SB203580, p38 inhibitor; SP600125, JNK inhibitor; PD98059, ERK inhibitor; DCF, 2′,7′-dichlorofluorescein.