Figure 7.

Activation of MK2 induces ROS production mediating glial cell apoptosis. The dominant active form of recombinant adenoviral p38 or dominant negative MK2 and MK5 recombinant adenoviral were transfected into F98 cells before exposure to heat stress or control heat treatment, and the cells received further incubation for 12 h at 37°C. (A and B) ROS quantity was assessed using 2′,7′-dichlorodihydrofluorescein diacetate staining; (A) images were obtained via laser scanning confocal microscopy and (B) analysis of the fluorescence intensity of ROS probes was performed by flow cytometry. (C) Analysis of apoptosis was performed via flow cytometry utilizing Annexin V-fluorescein isothiocyanate/propidium iodide staining. ^#P<0.05 vs. HS group. *P<0.05 vs. control group. ROS, reactive oxygen species; MK, mitogen-activated protein kinase-activated protein kinase; DCF, 2′,7′-dichlorofluorescein; MKK6b, MAPK kinase 6b.