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. Author manuscript; available in PMC: 2018 Mar 24.
Published in final edited form as: J Nat Prod. 2017 Feb 24;80(3):659–669. doi: 10.1021/acs.jnatprod.6b01150

Figure 6.

Figure 6

(+)-Strebloside inhibits NF-κB activation. (A) OVCAR3 cells were transfected with pNF-κB-luc plasmids overnight, then co-treated with 100 nM (+)-strebloside with or without 10 ng/mL TNF-α for 4 h. Cell extracts were collected and analyzed. (B) OVCAR3 cells were transfected with pNF-κB-luc plasmids overnight, then co-treated with 100 nM digitoxin with or without 10 ng/ml TNF-α for 4 h. Cell extracts were collected and analyzed. ANOVA was performed for fold change to analyze significance compared to untreated. Data represented as means ± SEM (** p ≤0.01, *** p ≤0.001).