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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Biochem Pharmacol. 2016 Dec 22;127:34–45. doi: 10.1016/j.bcp.2016.12.014

Fig. 4.

Fig. 4

Identification of C16, C20 and C159 as sites of S-nitrosylation on RhoA. (A) Recombinant RhoA was treated with or without Cys-NO (100 μM) for 30 min and the S-nitrosylation of RhoA was confirmed using the biotin-switch assay and Western blotting. (B–D) Biotinylated RhoA was analyzed by MS/MS and the spectrum of C16, C20 and C159-carbido-Biotin-BMCC tryptic peptides is shown with a summary of the molecular weights of these peptides (top right).