Figure 2.

Uptake study with OV-loaded NPs.

Notes: (A) The effect of antibody-decorated PLGA NPs on DC uptake assessed by flow cytometry. (a–d) Overlay of the histograms of untreated cells (red), cells treated with plain NPs (green), and cells treated with Ab-modified NPs (purple) for 0.18 iv COOH and 0.55 iv COOH groups with and without BS. (e–h) Overlay of the histograms representing DC uptake of 0.15 iv ester- and 0.55 iv ester-terminated NPs being formulated with and without BS. Histograms are representative of three individual experiments. (B) Bar diagram showing comparative fold increase in MFI of plain NPs and DCs treated with Ab-modified formulations for 0.18 iv COOH, 0.55 iv COOH, 0.15 iv ester, and 0.55 iv ester-terminated NPs, respectively. MFI for different NPs has been compared considering untreated cells (coumarin-6 only) as 1. All the studies were done in triplicate (p<0.05). (C) Representative overlapped CLSM images for coumarin-6 loaded NPs (0.18 iv COOH only) in DCs. Blue color represents the DAPI-stained nuclei which are encompassed by green fluorescence for the groups treated with coumarin-6-loaded NPs.

Abbreviations: Ab, antibody; AD, adsorption; BS, bis(sulfosuccinimidyl) suberate; C, COOH; CLSM, confocal laser scanning microscopy; COV, covalent; DAPI, 4′,6-diamidino-2-phenylindole; DC, dendritic cell; E, ester; iv, inherent viscosity; MFI, mean fluorescence intensity; NPs, nanoparticles; OV, ovalbumin; PLGA, poly-lactic-co-glycolide.