Table 2.
Formulation | Size ± SD (nm) | PDI ± SD | ZP ± SD (mV) | EE of OV ± SD (%) | OV loading ± SD (μg/mg) |
---|---|---|---|---|---|
0.18C-OV-NP | 150±5 | 0.22±0.89 | −22.55±0.32 | 45.68±1.01 | 63.21±2.11 |
0.55C-OV-NP | 156±2 | 0.21±0.11 | −24.76±0.42 | 46.21±3.11 | 62.56±3.60 |
0.15E-OV-NP | 170±6 | 0.32±0.03 | −20.11±0.32 | 41.86±0.98 | 67.18±3.50 |
0.55E-OV-NP | 171±3 | 0.31±0.06 | −19.11±0.87 | 42.13±2.54 | 70.33±2.80 |
0.18C-OVMP-NP | 141±2 | 0.26±0.01 | −19.01±0.56 | 39.01±1.35 | 53.11±1.44 |
0.55C-OVMP-NP | 145±5 | 0.32±0.03 | −26.21±0.25 | 45.73±2.91 | 59.27±1.21 |
0.15E-OVMP-NP | 151±3 | 0.36±0.06 | −19.16±0.15 | 41.45±1.46 | 57.16±2.98 |
0.55E-OVMP-NP | 152±6 | 0.21±0.07 | −20.01±0.61 | 52.62±5.22 | 62.64±1.64 |
0.18C-BS-OV-NP | 141±6 | 0.25±0.91 | −27.1±0.77 | 38.03±2.38 | 65.42±2.90 |
0.55C-BS-OV-NP | 143±2 | 0.31±0.04 | −24.02±0.07 | 40.55±1.25 | 65.91±0.52 |
0.15E-BS-OV-NP | 144±9 | 0.21±0.07 | −21.03±0.55 | 51.12±0.74 | 62.09±0.24 |
0.55E-BS-OV-NP | 163±8 | 0.31±0.09 | −19.1±0.48 | 48.13±0.91 | 68.48±2.27 |
0.18C-BS-OVMP-NP | 156±7 | 0.24±1.20 | −25.11±0.44 | 49.10±0.43 | 51.65±0.56 |
0.55C-BS-OVMP-NP | 159±4 | 0.21±0.98 | −24.11±0.35 | 55.42±0.21 | 54.11±4.15 |
0.15E-BS-OVMP-NP | 144±9 | 0.23±0.34 | −18.42±0.03 | 56.11±0.55 | 49.01±1.33 |
0.55E-BS-OVMP-NP | 151±2 | 0.36±0.14 | −19.45±0.56 | 59.15±1.56 | 51.35±0.46 |
Ab-0.18C-OV-NP | 323±3 | 0.31±0.09 | 1.31±0.17 | 31.13±1.10 | 39.91±1.21 |
Ab-0.55C-OV-NP | 330±1 | 0.36±0.02 | 2.95±0.18 | 33.75±0.91 | 40.14±0.19 |
Ab-0.15E-OV-NP | 376±5 | 0.30±0.17 | 4.54±0.21 | 30.15±1.01 | 32.57±0.74 |
Ab-0.55E-OV-NP | 379±2 | 0.30±0.03 | 3.32±0.43 | 30.23±2.90 | 49.58±0.47 |
Ab-0.18C-OVMP-NP | 413±6 | 0.31±0.9 | 3.13±0.11 | 39.04±1.25 | 42.15±1.52 |
Ab-0.55C-OVMP-NP | 461±3 | 0.31±0.05 | 2.91±0.34 | 35.53±2.21 | 44.11±1.46 |
Ab-0.15E-OVMP-NP | 421±1 | 0.28±0.41 | 2.97±0.34 | 35.21±1.15 | 39.16±2.21 |
Ab-0.55E-OVMP-NP | 486±4 | 0.34±0.25 | 4.25±0.52 | 42.56±2.56 | 40.15±3.11 |
Ab-0.18C-BS-OV-NP | 401±1 | 0.21±0.31 | 1.92±0.01 | 41.46±0.61 | 39.01±0.56 |
Ab-0.55C-BS-OV-NP | 501±9 | 0.23±0.21 | 1.45±0.15 | 40.42±0.36 | 40.15±0.62 |
Ab-0.15E-BS-OV-NP | 337±5 | 0.31±0.52 | 1.67±0.01 | 40.46±0.11 | 37.90±0.42 |
Ab-0.55E-BS-OV-NP | 487±3 | 0.21±0.62 | 3.51±0.03 | 45.24±0.09 | 42.15±0.55 |
Ab-0.18C-BS-OVMP-NP | 380±1 | 0.31±0.09 | 1.42±0.04 | 29.01±2.01 | 40.15±0.91 |
Ab-0.55C-BS-OVMP-NP | 419±4 | 0.29±0.05 | 1.99±0.13 | 34.11±0.23 | 42.43±0.55 |
Ab-0.15E-BS-OVMP-NP | 324±3 | 0.31±0.04 | 3.22±0.11 | 36.21±0.93 | 40.11±0.21 |
Ab-0.55E-BS-OVMP-NP | 407±9 | 0.39±0.06 | 1.87±0.02 | 39.01±0.14 | 41.45±0.91 |
Abbreviations: Ab, antibody; BS, bis(sulfosuccinimidyl) suberate; C, COOH; E, ester; EE, encapsulation efficiency; MP, monophosphoryl lipid A; NPs, nanoparticles; OV, ovalbumin; PDI, polydispersity index; SD, standard deviation; ZP, zeta potential.