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. 2018 Jan 11;13:367–386. doi: 10.2147/IJN.S144266

Table 2.

Particle size, PDI, ZP, EE of OV (%), and OV loading (µg/mg) of COOH and ester terminated NPs before and after Ab attachment (n=3)

Formulation Size ± SD (nm) PDI ± SD ZP ± SD (mV) EE of OV ± SD (%) OV loading ± SD (μg/mg)
0.18C-OV-NP 150±5 0.22±0.89 −22.55±0.32 45.68±1.01 63.21±2.11
0.55C-OV-NP 156±2 0.21±0.11 −24.76±0.42 46.21±3.11 62.56±3.60
0.15E-OV-NP 170±6 0.32±0.03 −20.11±0.32 41.86±0.98 67.18±3.50
0.55E-OV-NP 171±3 0.31±0.06 −19.11±0.87 42.13±2.54 70.33±2.80
0.18C-OVMP-NP 141±2 0.26±0.01 −19.01±0.56 39.01±1.35 53.11±1.44
0.55C-OVMP-NP 145±5 0.32±0.03 −26.21±0.25 45.73±2.91 59.27±1.21
0.15E-OVMP-NP 151±3 0.36±0.06 −19.16±0.15 41.45±1.46 57.16±2.98
0.55E-OVMP-NP 152±6 0.21±0.07 −20.01±0.61 52.62±5.22 62.64±1.64
0.18C-BS-OV-NP 141±6 0.25±0.91 −27.1±0.77 38.03±2.38 65.42±2.90
0.55C-BS-OV-NP 143±2 0.31±0.04 −24.02±0.07 40.55±1.25 65.91±0.52
0.15E-BS-OV-NP 144±9 0.21±0.07 −21.03±0.55 51.12±0.74 62.09±0.24
0.55E-BS-OV-NP 163±8 0.31±0.09 −19.1±0.48 48.13±0.91 68.48±2.27
0.18C-BS-OVMP-NP 156±7 0.24±1.20 −25.11±0.44 49.10±0.43 51.65±0.56
0.55C-BS-OVMP-NP 159±4 0.21±0.98 −24.11±0.35 55.42±0.21 54.11±4.15
0.15E-BS-OVMP-NP 144±9 0.23±0.34 −18.42±0.03 56.11±0.55 49.01±1.33
0.55E-BS-OVMP-NP 151±2 0.36±0.14 −19.45±0.56 59.15±1.56 51.35±0.46
Ab-0.18C-OV-NP 323±3 0.31±0.09 1.31±0.17 31.13±1.10 39.91±1.21
Ab-0.55C-OV-NP 330±1 0.36±0.02 2.95±0.18 33.75±0.91 40.14±0.19
Ab-0.15E-OV-NP 376±5 0.30±0.17 4.54±0.21 30.15±1.01 32.57±0.74
Ab-0.55E-OV-NP 379±2 0.30±0.03 3.32±0.43 30.23±2.90 49.58±0.47
Ab-0.18C-OVMP-NP 413±6 0.31±0.9 3.13±0.11 39.04±1.25 42.15±1.52
Ab-0.55C-OVMP-NP 461±3 0.31±0.05 2.91±0.34 35.53±2.21 44.11±1.46
Ab-0.15E-OVMP-NP 421±1 0.28±0.41 2.97±0.34 35.21±1.15 39.16±2.21
Ab-0.55E-OVMP-NP 486±4 0.34±0.25 4.25±0.52 42.56±2.56 40.15±3.11
Ab-0.18C-BS-OV-NP 401±1 0.21±0.31 1.92±0.01 41.46±0.61 39.01±0.56
Ab-0.55C-BS-OV-NP 501±9 0.23±0.21 1.45±0.15 40.42±0.36 40.15±0.62
Ab-0.15E-BS-OV-NP 337±5 0.31±0.52 1.67±0.01 40.46±0.11 37.90±0.42
Ab-0.55E-BS-OV-NP 487±3 0.21±0.62 3.51±0.03 45.24±0.09 42.15±0.55
Ab-0.18C-BS-OVMP-NP 380±1 0.31±0.09 1.42±0.04 29.01±2.01 40.15±0.91
Ab-0.55C-BS-OVMP-NP 419±4 0.29±0.05 1.99±0.13 34.11±0.23 42.43±0.55
Ab-0.15E-BS-OVMP-NP 324±3 0.31±0.04 3.22±0.11 36.21±0.93 40.11±0.21
Ab-0.55E-BS-OVMP-NP 407±9 0.39±0.06 1.87±0.02 39.01±0.14 41.45±0.91

Abbreviations: Ab, antibody; BS, bis(sulfosuccinimidyl) suberate; C, COOH; E, ester; EE, encapsulation efficiency; MP, monophosphoryl lipid A; NPs, nanoparticles; OV, ovalbumin; PDI, polydispersity index; SD, standard deviation; ZP, zeta potential.