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. Author manuscript; available in PMC: 2018 Feb 9.
Published in final edited form as: Nature. 2017 Aug 9;548(7667):347–351. doi: 10.1038/nature23318

Figure 3. Short poly(A) tail length and TUT4/7-mediated oligo-uridylation target oocyte transcripts for degradation.

Figure 3

a, Expression scatterplot showing relative average expression of transcripts between Tut4/7CTL and Tut4/7cKO germinal vesicle (GV) oocytes. Significantly deregulated (p < 0.01) genes with a fold change greater than 2 are highlighted in red. b, Enrichment and depletion analysis of Tut4/7cKO upregulated transcripts in gene expression clusters. The p-values for enrichment are indicated on the top blue bar and those for depletion on the bottom red bar. The hyper-geometric test was used to establish the significance. n represents the number of transcripts defining the cluster and x the number of Tut4/7cKO upregulated transcripts within the cluster. c, Frequency of transcripts plotted against poly(A) tail length (8-78 nucleotides) in Tut4/7CTL and Tut4/7cKO germinal vesicle (GV) oocyte. Transcripts upregulated and not upregulated in Tut4/7cKO oocytes are indicated in red and black, respectively. Data points and horizontal lines represent biological replicates’ mean and range, respectively. The number of transcripts / number of genes / percentage of transcripts with poly(A) tail length shorter than 79 nucleotides are 1,020,715 / 11,160 / 35% for Tut4/7CTL (not upregulated), 14,925 / 598 / 36% for Tut4/7CTL (upregulated), 173,641 / 8,790 / 30 % for Tut4/7cKO (not upregulated), and 6,223 / 549 / 46% for Tut4/7cKO (upregulated). d, Quantification of mRNA 3′ oligo-uridylation of transcripts upregulated and not upregulated in Tut4/7cKO oocytes with short (≤ 30 nucleotides) poly(A) tails from Tut4/7CTL and Tut4/7cKO GV oocytes. The numbers of oligo-uridylated transcripts and in parentheses the number of genes represented by these transcripts are shown. The bars’ heights and vertical lines indicate the mean and range, respectively. e, Ratio of oligo- to mono-uridylation for transcripts with short (≤ 30 nucleotides) poly(A) tails from Tut4/7CTL and Tut4/7cKO GV oocytes. The fold change and the significance are indicated (one-tailed t-test; *, p < 0.05) (d, e). The data for the Tut4/7CTL samples are derived from the libraries presented in Fig. 1.