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. Author manuscript; available in PMC: 2018 Apr 25.
Published in final edited form as: Nature. 2017 Oct 25;551(7678):110–114. doi: 10.1038/nature24293

Extended Data Figure 3. Mechanism of IL-1R8-dependent regulation of NK cells.

Extended Data Figure 3

(a) Splenic CD27low NK cell frequency in wild type, Il1r8-/-, Il18-/-, and Il18-/-/Il1r8-/- mice.

(b) Peripheral CD27low NK cell frequency in wild-type, Il1r8-/-, Il1r1-/- and Il1r8-/-Il1r1-/- mice (left) and IFNγ production by splenic NK cells after IL-12 and IL-1β or IL-18 stimulation (right).

(c, d) Splenic CD27low NK cell frequency in Il1r8+/+ and Il1r8-/- mice upon commensal flora depletion (c) and breeding in co-housing conditions (d).

(e) STED microscopy of human NK cells stimulated with IL-18. Magnification bar: 2μm.

(a-d) *p < 0.05, **p < 0.01, ***p < 0.001 between selected relevant comparisons, two-tailed unpaired Student’s t test; Centre values and error bars represent mean ± SEM. a, n= 3, 5, or 6 mice; at least 5 animals per group were used (b-d). a-d: one experiment was performed. e: representative images out of three collected from two donors.