Skip to main content
. 2017 Nov 28;8(69):113583–113597. doi: 10.18632/oncotarget.22745

Figure 3. Effect of ARF6, NEDD9 and MT1-MMP silencing on protein expression and invasiveness of malignant cells.

Figure 3

Cells were transfected with ARF6, NEDD9 and MT1-MMP siRNAs (25 nmol/L) or scrambled siRNA (25 mmol/L) using Dharmafect. (A) Expression of ARF6, NEDD9 and MT1-MMP was analyzed by Western blot analysis. The values above the bands represent relative density of the bands normalized to β-actin. (B) Cells invasive potential was evaluated by using Matrigel Invasion Chambers. Data shown are mean ± SEM of values from 3 independent experiments. Statistically significant changes in the ARF6, NEDD9 and MT1-MMP knockdown groups compared to their respective control groups are shown by asterisk (P < 0.05).