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. 2017 Nov 20;8(69):113701–113718. doi: 10.18632/oncotarget.22555

Figure 5. XIST inversely mutual-regulates miR-140/miR-124.

Figure 5

(A) The expression of miR-140 in BxPC-3 and PANC-1 cells in response to XIST knockdown was determined by using real-time PCR assays. (B) The expression of miR-124 in BxPC-3 and PANC-1 cells in response to XIST knockdown was determined by using real-time PCR assays. (C) miR-140/miR-124 mimics were transfected into BxPC-3 and PANC-1 cells to achieve miR-140 and miR-124 overexpression, respectively. (D) miR-140/miR-124 inhibitor was transfected into BxPC-3 and PANC-1 cells to achieve miR-140 and miR-124 inhibition, respectively. (E) miR-140 mimics or miR-140 inhibitor was transfected into BxPC-3 and PANC-1 cells; XIST expression in BxPC-3 and PANC-1 cells in response to miR-140 overexpression or inhibition was determined by using real-time PCR assays. (F) miR-124 mimics or miR-124 inhibitor was transfected into BxPC-3 and PANC-1 cells; XIST expression in BxPC-3 and PANC-1 cells in response to miR-124 overexpression or inhibition was determined by using real-time PCR assays. The data are shown as mean± SD of three independent experiments. *P<0.05, **P<0.01.