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(A) Flow cytometry was performed to analyze the CD44+/CD24-/low phenotype of BCSCs in T-47D and SKBR3 cell lines. (B) T-47D and SKBR3 CSCs and non-CSCs were treated with different concentrations of 5-FU (0∼20 μM). Forty-eight hours later, MTT assays were performed to evaluate the sensitivity of T-47D and SKBR3 CSCs and non-CSCs to 5-FU treatment. *P < 0.05.
