Figure 2. Expression of miR-489 was decreased in BCSCs.

(A) QRT-PCR assays were performed to detect the relative expression of miR-489 in T-47D and SKBR3 CSCs and non-CSCs. ^*P < 0.05. (B) Breast cancer patients’ tissues (n = 30) were digested by collagenase type III. CSCs and non-CSCs in cell suspension were separated flow cytometry. Subsequently, relative expression of miR-489 in these CSCs and non-CSCs was detected by qRT-PCR analysis. ^*P < 0.05.