Figure 4. Metabolic response and choline kinase status of A2780CisR xenografts following 2 weeks of treatment with vistusertib.

(A) alone, paclitaxel (P) alone, the combination of vistusertib and paclitaxel (A+P) and vehicle (V). Choline kinase expressions were measured by western blotting and β-actin is used as a loading control. Tumor phospholipid and bioenergetic metabolites were measured by ³¹P-MRS of tumor extracts. Data are expressed as median and interquartile ranges (25% and 75%). ^* p < 0.05; minimum n = 7 in each treatment group.