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. 2017 Dec 9;8(69):114072–114080. doi: 10.18632/oncotarget.23120

Figure 4. UBE2N was identified as miR-147b target.

Figure 4

(A) UBE2N gene was predicted as the potential miR-147b target predicted by several programs. (B) MiR-147b binding elements in the 3’-UTR of UBE2N and the mutated sites. (C) Luciferase reporter analysis of direct binding of miR-147b to the 3’-UTR of UBE2N. (D-E) mRNA expression levels of UBE2N in clinical samples (D) and HCC cell lines (E). (F-G) After transfected with scramble control, miR-147b mimic and anti-miR-147b oligoes, protein levels of UBE2N in HepG2 (F) and Huh 7 (G) cell lines were detected with specific antibody. (H) Protein level of UBE2N in adjacent tissues and HCC tumor tissues were analyzed by western blot. **P<0.01, ***P<0.001.