Skip to main content
. 2017 Dec 11;8(69):114210–114225. doi: 10.18632/oncotarget.23174

Figure 7. The SHH pathway is upregulated in an additional CNS HGNET-BCOR case.

Figure 7

(A) The sequence of exon 15 of BCOR was analyzed by PCR in the genomic DNA extracted from the blood of P1 (lane 1), the primary tumor of P2 (lane 2) or from the primary tumor of P1 (lane 3). The sizes of the wt BCOR and of the BCOR ITDs are indicated. (B) Protein sequence of the BCOR allele of P2 carrying the ITD compared to the wild type BCOR and to the BCOR ITD detected in P1. (C) qRT-PCR analysis was performed using primers recognizing BCOR, GLI2, PTCH1 or GLI1. After normalization to the housekeeping gene HPRT1, the fold change of the expression of P2 with respect to P1 was calculated. Expression analysis was done in triplicates. Expression analysis was done on RNA extracted from FFPE material.