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(A-C) The relative mRNA and protein expression levels of E-cadherin were measured by RT-qPCR and western blot analysis, respectively. (D-E) E-cadherin (red) localization and expression were assessed by immunofluorescent analysis in NC, miR-424 mimics, ASO-miR-424 or ASO-negative control (NC) transfected HEC-1A and Ishikawa cells.
