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. 2017 Dec 13;8(69):114281–114291. doi: 10.18632/oncotarget.23218

Figure 4. E2F6 gene 3’-UTR carries a putative miR-424 binding site and is negatively regulated by miR-424.

Figure 4

(A) a1. Bioinformatics analysis predicted that E2F6 is the target gene of hsa-miR-424. a2. Construction of pmirGLO-luciferase reporter vectors (wild-type and mutant). (B) Luciferase reporter vectors confirmed that miR-424 can bind directly to the 3’-UTR of E2F6 mRNA. (C) The relative E2F6 mRNA expression level is regulated by miR-424. (D) The relative E2F6 protein expression level is regulated by miR-424, as determined by western blot analysis. (ASO: antisense oligonucleotides; NC: negative control.) All error bars indicate the means ± SD. Experiments were performed in triplicate. *P < 0.05 compared with the corresponding control group.