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. 2017 Dec 13;8(69):114281–114291. doi: 10.18632/oncotarget.23218

Figure 7. E2F6 regulates miR-424 transcription in EC cells.

Figure 7

(A) Landscape of miR-424 transcription factor from UCSC data. (B) Left, schematic presentation of luciferase vectors, a series of deletion mutant vectors. Right, relative luciferase activity constructs. HEC-1A cells were incubated with the E2F6-overexpression plasmid and luciferase reporter. (C) Relative luciferase intensity was detected in EC cells after transfection with firefly luciferase constructs and pcDNA3.1, pcDNA3.1/E2F6, si-NC or siE2F6 for 24 h. (D) RT-PCR was used to detect the relative expression of miR-424 levels in HEC-1A cells transfected with pcDNA3.1, pcDNA3.1/E2F6, si-NC or siE2F6. All error bars indicate the means ± SD. Experiments were performed in triplicate. *P < 0.05 compared with the corresponding control group.