Table 1. Oligonucleotide sequences of the primers and siRNAs.

Genes	Oligonucleotide sequences	Protocols
Primers
TNF-a	Forward: 5′-CCGTCTCCTACCAGACCAAGG-3′
	Reverse: 5′-CTGGAAGACCCCTCCCAGATAG-3′
IL-1b	Forward: 5′-CTGATGGCCCTAAACAGATGAAG-3′
	Reverse: 5′-GGTCGGAGATTCGTAGCAGCTGGAT-3′
IL-10	Forward: 5′-CATGCTGCTGGGCCTGAA-3′
	Reverse: 5′-CGTCTCCTTGATCTGCTTGATG-3′
Sirt6	Forward: 5′-GCCCCGCTTCCGGCGGAAG-3′
	Reverse: 5′-ATTGTTTTTATTGCATTGAGGAC-3′
Drosha	Forward: 5′-TCCCTGCTGCCCAAGATTTC-3′	stage1: 95°C, 30 sec
	Reverse: 5′-CAGGGCTTTGCTGCACCTTA-3′	stage2: 95°C, 5 sec
Sirt1	Forward: 5′-TGTGGTAGAGCTTGCATTGATCTT-3′	55°C, 30 sec
	Reverse: 5′-GGCCTGTTGCTCTCCTCATT-3′	72°C, 35 sec
GAPDH	Forward: 5′-AAGGTGAAGGTCGGAGTCA-3′	40 cycles
	Reverse: 5′-GGAAGATGGTGATGGGATTT-3′
siRNA
Sirt6	Sense: 5′-CCCUGGUCUCCAGCUUAAATT-3′
	Anti-Sense: 5′-UUUAAGCUGGAGACCAGGGTT-3′
Sirt1	Sense: 5′-GATTATTGCCGGAAACAA-3′
	Anti-Sense: 5′-TCCTTTCAGAACCACCAAA-3′
Drosha	Sense: 5′-GGATTAGCAACCTATCGGA-3′
	Anti-Sense: 5′-AAGGACCAAGTATTCAGCA-3′
GAPDH:	Sense: 5′-GUAUGACAACAGCCUCAAGTT-3′
	Anti-Sense: 5′-CUUGAGGCUGUUGUCAUACTT-3′