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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: J Immunol. 2017 Sep 8;199(8):2680–2691. doi: 10.4049/jimmunol.1700792

Figure 6. Encephalitogenicity of a NFM T cell line is concomitant with infiltration of tetramer positive MOG specific T cell in the CNS.

Figure 6

A) Lymph nodes from NFM15-35 primed C57BL/6 wild type mice were harvested and cultured for 1 week on NFM15-35. 2D micropipette adhesion frequency assay was used to assess MOG versus NFM specificity. This figure represents one of 2 experiments, displaying data from 36 cells. B) Irradiated (400 rads) C57BL/6 mice received 10×106 cells from a NFM T cell line (i.p.). 25 days post transfer, the CNS of mice with scores from 1.0 to 3.5 were harvested and antigen specificity was quantitated with MOG38-49 or NFM18-30 tetramer (C). D) Representative flow plots from adoptive transfer of a Thy1.1+ NFM T cell line in to a Thy1.2 recipient. C & D are data representative from 1 of 2 experiments. All statistics were done using 2-tailed unpaired, parametric t tests assuming both populations had equal standard deviations.