Figure 3. Hindsight expression in stage-14 follicle cells is essential for normal ovulation.

(A–D) Hnt expression (green) in control (A) and hnt^RNAi (B–C) stage-14C egg chambers with FC1. FC1 expression (FC1 > RFP) is shown in red. Inserts are high magnification of Hnt expression in squared areas. The quantification of Hnt expression (categorized as high-Hnt, low-Hnt, A/P-Hnt, and None-Hnt) in stage-14C egg chambers is shown in (D). The number of stage-14C egg chambers (selected according to no nurse-cell nuclei/high FC1 expression) analyzed is noted above each bar. (E–H) Hnt expression (green) in control (E) and hnt^RNAi (F–G) stage-14C egg chambers with FC2. FC2 expression (FC2 > RFP) is shown in red. Inserts are high magnification of Hnt expression in squared areas. The quantification of Hnt expression in stage-14C egg chambers is shown in (H). The stage-14C egg chambers are selected according to no nurse-cell nuclei/high FC2 expression. (I–J) The quantification of egg laying (I) and mature egg chambers in each female’s ovaries after egg laying (J) in control or hnt^RNAi females with FC1 (green bars) or FC2 Gal4 (blue bars). The number of females is noted above each bar. (K–L) The egg-laying time in control or hnt^RNAi females with FC1 (K) or FC2 (L). Also see Supplementary file 1. *p<0.05, **p<0.01, ***p<0.001.

Figure 3—figure supplement 1. Hnt expression in stage-14A and stage-14B egg chambers.

(A–H) Hnt expression (green in A-C and E-G) in stage-14A (A–D) and stage-14B (E–H) egg chambers with FC1 driving RFP (control, A and E) or hnt^RNAi (B–C and F–G). Quantification of Hnt expression is shown in (D) and (H). (I–P) similar as in (A–H) but using FC2 Gal4 instead.

Figure 3—figure supplement 2. hnt depletion in stage-14 follicle cells does not affect oogenesis in virgin females.

(A–B) Quantification of stage-14 egg chambers in virgin females before egg laying experiment. (A) is using FC1 Gal4, while (B) is using FC2-Gal4.