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. 2017 Dec 19;6:e29887. doi: 10.7554/eLife.29887

Figure 4. Hindsight is required for OA-induced follicle rupture.

(A–D) Representative images show control (A) and hntRNAi (B–C) egg chambers with FC1 after three-hour culture with 20 μM OA. Quantification of OA-induced follicle rupture is shown in (D). (E–H) Representative images show control (E) and hntRNAi (F–G) egg chambers with FC2 after three-hour culture with 20 μM OA. Quantification is shown in (H). Egg chambers were isolated according to FC1 > RFP (red in A-C) or FC2 > RFP (red in E-G) expression. Bright-field images of the egg chambers are shown in blue, and ruptured egg chambers are marked by white arrowheads. The number of egg chambers is listed above each bar. ***p<0.001.

Figure 4.

Figure 4—figure supplement 1. hnt mutant mature follicles are defective for OA-induced follicle rupture.

Figure 4—figure supplement 1.

(A–C) Representative images show hntpeb/+ (A), hntpeb/hntXE81 (B), and hntpeb/hntEH704a (C) mature follicles after three-hour culture with OA. Mature follicles were isolated according to FC2 > RFP expression showing in red. Bright-field images of the egg chambers are shown in blue, and ruptured follicles are marked by white arrowheads. (D) Quantification of OA-induced rupture is shown. ***p<0.001.