Skip to main content
. 2017 Dec 22;6:e31101. doi: 10.7554/eLife.31101

Appendix 1—table 1. Gene ontology analysis.

5 hr 16 hr
0.1 kPa Receptor-mediated endocytosis Cell differentiation
** receptor-mediated endocytosis * forebrain neuron fate commitment
* endocytic adaptor activity Cytoskeleton
* clathrin adaptor activity ** intermediate filament binding
** regulation of receptor recycling * protein localization to cytoskeleton
*** positive regulation of
receptor recycling		 * protein localization to
microtubule cytoskeleton
Vesicle transport Cell aggregation
** endosomal vesicle fusion * cell aggregation
* vesicle-mediated transport GTPase signaling pathway
*** response to lipid ** G-protein coupled neurotensin
receptor activity
0.1 kPa External stimulus
*** response to external stimulus
** regulation of cell-substrate
adhesion
* laminin-1 binding
Neuronal development
* axon midline choice
point recognition
** regulation of long term
synaptic depression
Cell polarity
* maintenance of cell polarity
* positive regulation of Wnt
signaling pathway, planar
cell polarity pathway
0.1 kPa Cell migration
** regulation of cell migration
* positive regulation of cell migration
** regulation of cell motility
* positive regulation of cell motility
GTPase signaling pathway
** regulation of Rho-dependent
protein serine/threonine
kinase activity
* adenylate cyclase-activating
G-protein coupled receptor
signalling pathway
Cytoskeleton
* neurofilament cytoskeleton
20 kPa Nervous system development Nervous system development
* central nervous system development * central nervous system
neuron development
* regulation of dendrite development ** negative regulation of neurogenesis
** regulation of neurogenesis * neuronal action potential propagation
** regulation of nervous
system development		 ** regulation of neuron
projection development
* pyramidal neuron development * axon midline choice point recognition
* cell projection * axon guidance receptor activity
** internode region of axon Signaling transduction
** cellular developmental process * protein kinase A signaling
* regulation of cell development ** positive regulation of phosphatidylinositol
3-kinase signalling
20 kPa Cell differentiation *** second-messenger-mediated signaling
* cell differentiation Cell differentiation
* pyramidal neuron differentiation ** neuron fate commitment
* regulation of cell morphogenesis
involved in differentiation		 ** central nervous system
neuron differentiation
Vesicle transport ** negative regulation of
neuron differentiation
** late endosome Morphogenesis
* regulation of lipid transport * cell morphogenesis
Cell migration * positive regulation of dendritic
spine morphogenesis
20 kPa ** locomotory behavior Cell adhesion
* negative regulation of
neuron migration		 * response to mechanical stimulus
GTPase signaling pathway ** extracellular matrix binding
* Rho guanyl-nucleotide
exchange factor activity		 * positive regulation of
cell-substrate adhesion
** Rho protein signal transduction ** laminin-1 binding
* positive regulation of adenylate cyclase
activity involved in G-protein coupled
receptor signalling pathway		 **** positive regulation
of cell adhesion
Cytoskeleton ** protein binding involved in
cell adhesion
* regulation of actin
cytoskeleton organization		 Cell migration
20 kPa * regulation of actin
filament-based process		 ** positive regulation of
cell migration
* negative regulation of cell adhesion
involved in substrate-bound
cell migration
** positive regulation of locomotion
Neuronal regeneration
* negative regulation of
axon regeneration
* negative regulation of
neuron projection regeneration
Transport
** regulation of transport
20 kPa Cytoskeleton
* actin filament binding
** actin filament bundle assembly
*p<0.05; **p<0.01;
***p<0.001; ****p<0.0001. t test		 * ruffle assembly