Figure 3.

Chromatogram obtained by combining protein A and size-exclusion HPLC columns for the analysis of monoclonal antibodies versus other components in a Chinese hamster ovary (CHO) cell culture. The peaks for the monoclonal antibodies and their aggregates are shown to the right, after their elution from the protein A column and loading onto the size-exclusion column, and the results for the other sample components (e.g., DNA fragments, host cell proteins, amino acids, nucleotides, etc.) that were non-retained by protein A column are shown to the left, after the first application of the loading buffer and passage of these components through the size-exclusion column. Adapted with permission from Ref. 64.