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. 2017 Dec 5;113(11):2519–2529. doi: 10.1016/j.bpj.2017.09.028

Figure 3.

Figure 3

The extracellular domain of Dsg3 is ordered. Shown here are HaCaT cells expressing (a) Dsg3-ΔEA-GFP and (b) Dsg3-link-GFP. The average intensity image and individual ROIs (white rectangle) at each excitation polarization are shown. The intensity from a single pixel is plotted as a function of excitation polarization and fit to the sinusoid (Eq. 1) (solid line). Desmosome order factor is shown as a masked heatmap. Pixel-by-pixel order factor is plotted as a function of S/B. (c and d) Given here is an average intensity image of the HaCaT cell expressing (c) mem-GFP and (d) GFP. Average intensity and order factor heatmap are shown for the ROI. The pixel-by-pixel order factor is plotted over S/B. (a–d) Scale bars, 10 μm; ROI scale bars, 2 μm. (e) Shown here is the mean order factor as a function of mean S/B for individual cells expressing each construct (error bars: SD) compared to the theoretical maximum order factor (red line) and disorder threshold (blue line). (f) Box plots of cell population order factor; full range (whiskers) are given with the median (line) and 25–75 percentile range (box). (∗∗∗∗p < 0.0001; ns = no significance, by one-way ANOVA.) To see this figure in color, go online.