Figure 1.

Application of SMLM clustering algorithms to PALM data of FAs. (A) Given here is a PALM image of a fixed REF cell expressing integrin β3 labeled with mEos2. (B) Given here is a zoom-in PALM image corresponding to the green rectangle in (A). (C) Given here is a scatter plot of the mEos2 localizations corresponding to the green rectangle in (A). (D) Given here is Ripley’s L(r)-r as a function of r, obtained from the localizations in (C). (E) Shown here are clusters obtained from the localizations in (C) by DBSCAN. The minimum number of localizations was set to 10, and two values were chosen for the maximum search radius r_max: 0.05 and 0.10 μm. The different colors of the localizations indicate to which cluster they belong; the background localizations are red. (F) Shown here is a result of EMGM analysis of the localizations in (C). The red dots symbolize the localizations, and the blue ellipses the 2σ error ellipses of the components. (G) Histograms show the eccentricity b/a, localization density, number of localizations, and area πab of the 2σ error ellipses of the components obtained by EMGM from the complete PALM data set in (A). The rightmost bins in each histogram (except for the eccentricity histogram) contain all values within that bin and larger.