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. 2017 Dec 5;113(11):2336–2343. doi: 10.1016/j.bpj.2017.10.002

Figure 1.

Figure 1

The chemical structures of Cy3 and Cy5 (left), and the sequence of the 33 nt stem-loop derived from 5S rRNA (right). Four forms of the RNA have been studied, with the four possible terminal basepairs. The fluorophores are connected to the 5′-terminus of the RNA (Cy) via one of the C3 linkers attached to the indole nitrogen atom.