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. 2017 Aug 4;25(12):2705–2714. doi: 10.1016/j.ymthe.2017.07.018

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Mechanism of Action of CEBPA-51 in HepG2 Cells

(A) Western blot after co-immunoprecipitation of Ago1–4 and biotinylated CEBPA-51. (B) qPCR for CEBPA mRNA after transfection of 10 nM CEBPA-51 wild-type and Ago2 knockout MEFs. (C) qPCR at the CEBPA locus after chromatin immunoprecipitation with biotinylated CEBPA-51. The CEBPA-51 target site is approximately 3 kb downstream of the CEBPA TSS. (D) qPCR for CTR9 mRNA after transfection with CTR9 siRNA and qPCR for CEBPA mRNA after co-transfection of CTR9 siRNA and CEBPA-51. Statistical significance shown for the indicated condition compared to FLUC transfection: **p < 0.01. Error bars represent SEM.