Figure 3.

GNeo-IDUA Reduces the NRE Heparan Sulfate Biomarker in the MPSI Mouse Brain

(A) Idua^−/− mice were treated intranasally with 1 mg/kg IDUA or GNeo-IDUA every other day for 14, 30, and 60 days. n = 10–12 mice for 14 days and 30 days; n = 5–6 mice for 60 days. (B) Idua^−/− mice were treated intranasally with 1 mg/kg IDUA or GNeo-IDUA twice a day for 14 days. n = 7–10 mice per treatment group from two separate experiments. Non-reducing end biomarker was determined in heparan sulfate isolated from whole brains. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars indicate SEM, and significance was determined by one-way analysis of variance (ANOVA) with Tukey’s post-hoc analysis.