Table 1.

Analysis of Specific and Collateral Packaging of rAAV5-UF26 in HEK293 and Sf9 Cells

Reference Genome	NGS Library
	PCR-free	PCR
HEK293
UF26 cassette	96.50	95.24
	96.59	95.38
Vector backbone	2.58	3.49
	2.52	3.39
pHelper	0.04	0.04
	0.04	0.04
rep2cap5	0.71	1.00
	0.68	0.97
H. sapiens	0.17	0.22
	0.17	0.22
Sf9
UF26 cassette	99.38	99.15
	99.39	99.20
Vector backbonea	0.30	0.41
	0.30	0.41
Baculovirus/Bacmidb	0.29	0.39
	0.27	0.34
rep2/cap5c	0.014	0.003
	0.015	0.003
S. frugiperda	0.025	0.040
	0.025	0.040

Relative content of DNA in purified virions is shown as percentage of total sequences identified by NGS analysis. All NGS analyses were conducted in duplicates shown for each reference genome in two parallel rows. See also Figures S5–S7 and Tables S4 and S5.

^aIncluding Gen^R.

^bAcMNPV(strain_E2), miniF, LacZ, and Kan^R.

^cpIR-VPm11-hR2-900, pIR-rep78polHind-hr2.